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Influenza B virus Nucleoprotein antibody [8C8]

Anti-Influenza B virus Nucleoprotein antibody [8C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00707

Cat. No. GTX00707

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8C8

Isotype

 IgG1

Application

 ICC/IF, IP, ELISA

Reactivity

 Influenza B virus
Package
100 μg ($399)
See all Influenza B virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100
IP 1:100
ELISA Assay dependent
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Product Note

This antibody reacts with NP of all Influenza B viruses so far tested (113 clinical strains), including Yamagata lineage strains; Mie/1/1993, JohanesBurg/5/1999, Florida/4/2006 and Victoria lineage strains; Lee/1940, Gif/21/1973, Shangdong/7/1997, Malasia/2506/2004, Massachustts/2/2012. No cross reactivity with influenza A viruses.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group.

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza B Virus Nucleoprotein , Influenza B Virus NP

Database

Research Area

DATA IMAGES

Anti-Influenza B virus Nucleoprotein antibody [8C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00707

GTX00707 ICC/IF Image

ICC/IF analysis of MDCK cells infected with Influenza B virus using GTX00707 Influenza B virus Nucleoprotein antibody [8C8]
Upper : The cells infected with an Influenza B virus vaccine strain, Malaysia/2506/2004 as a representative of Victoria group.
Lower : The cells infected with an Influenza B virus vaccine strain, Florida/4/2006 as Yamagata group.
Fixation : 4% PFA in PBS
Permeabilization : 0.1% Triton X-100 in PBS

REFERENCE

Clone Reference

Nakagawa N et al. J Virol Methods.? 1999; 79 (1):113-20 Rapid detection and identification of two lineages of influenza B strains with monoclonal antibodies.
Application : ICC/IF, IP
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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399