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Integrin beta 1 / CD29 antibody

KO/KD
GTX128839_41374_20170309_WB_KO_watermark_18121410_972.jpg
GTX128839_41374_IHC_2_18121410_433.jpg
GTX128839_41374_WB_R_18121410_425.jpg
GTX128839_41374_WB_M_18121410_610.jpg
GTX128839_41374_WB_M_2_18121410_713.jpg
GTX128839_41374_IHC_18121410_181.jpg
GTX128839_41374_20150430_IP_18121410_459.jpg
GTX128839_42760_20170216_WB_18121410_923.jpg
GTX128839_42760_20170216_WB_2_18121410_417.jpg

Cat No. GTX128839

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

A431 , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2 , *J82

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GPIIA Antibody, VLA-BETA Antibody, ITGB1 Antibody, VLAB Antibody, integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) Antibody, MDF2 Antibody, FNRB Antibody, CD29 Antibody, MSK12 Antibody

Cellular Localization

Cell membrane,Cleavage furrow,Melanosome

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX128839_41374_20170309_WB_KO_watermark_18121410_972.jpg

GTX128839 WB Image

KO/KD

Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX128839_41374_IHC_2_18121410_433.jpg

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128839_41374_WB_R_18121410_425.jpg

GTX128839 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128839_41374_WB_M_18121410_610.jpg

GTX128839 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128839_41374_WB_M_2_18121410_713.jpg

GTX128839 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg BCL-1 whole cell lysate/extract
C. 30 µg Raw264.7 whole cell lysate/extract
D. 30 µg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128839_41374_IHC_18121410_181.jpg

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128839_41374_20150430_IP_18121410_459.jpg

GTX128839 IP Image

Immunoprecipitation of Integrin beta 1 / CD29 protein from HeLa whole cell extracts using 5 μg of Integrin beta 1 / CD29 antibody (GTX128839).
Western blot analysis was performed using Integrin beta 1 / CD29 antibody (GTX128839).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX128839_42760_20170216_WB_18121410_923.jpg

GTX128839 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000.

GTX128839_42760_20170216_WB_2_18121410_417.jpg

GTX128839 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000.

REFERENCE
REVIEW

Integrin beta 1 / CD29 antibody Cat No. GTX128839

Rating ( Average 4 based on 1 users reviews)
Application
Immunoprecipitation(IP) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 13-Dec-2013
Score :
Application Tested : IP
Sample Species : Hu
Sample : J82 cells
Amount used : 500μg
Blocking : 5% BSA, 25°C, 2Hr
Fixation : NP-40 lysis buffer
Primary Antibody : 1:10000, 4°C, overnightHr
Figure Note : 1 ug for IP
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169