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Interferon gamma Receptor 1 antibody

KO/KD Orthogonal
Anti-Interferon gamma Receptor 1 antibody used in Western Blot (WB). GTX103098
Anti-Interferon gamma Receptor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103098
Anti-Interferon gamma Receptor 1 antibody used in Western Blot (WB). GTX103098
Anti-Interferon gamma Receptor 1 antibody used in Western Blot (WB). GTX103098
Anti-Interferon gamma Receptor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103098

Cat. No. GTX103098

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Interferon gamma Receptor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

THP-1 , HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Interferon gamma Receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615520

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon gamma receptor 1 , CD119 , IFNGR , IMD27A , IMD27B

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Interferon gamma Receptor 1 antibody used in Western Blot (WB). GTX103098

GTX103098 WB Image

KO/KD

Wild-type (WT) and Interferon gamma Receptor 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Interferon gamma Receptor 1 antibody (GTX103098) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Interferon gamma Receptor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103098

GTX103098 ICC/IF Image

Interferon gamma Receptor 1 antibody detects Interferon gamma Receptor 1 protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Interferon gamma Receptor 1 stained by Interferon gamma Receptor 1 antibody (GTX103098) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Interferon gamma Receptor 1 antibody used in Western Blot (WB). GTX103098

GTX103098 WB Image

Orthogonal

HepG2 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Interferon gamma Receptor 1 antibody (GTX103098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Interferon gamma Receptor 1 antibody used in Western Blot (WB). GTX103098

GTX103098 WB Image

Interferon gamma Receptor 1 antibody detects Interferon gamma Receptor 1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Interferon gamma Receptor 1 antibody (GTX103098) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Interferon gamma Receptor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103098

GTX103098 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Interferon gamma Receptor 1(GTX103098) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kim DY et al. Biochem Cell Biol 2019; (Epub) Mitochondrial dysfunction regulates the JAK/STAT pathway by LKB1-mediated AMPK activation in an ER stress-independent manner.
Application : WB
Reactivity : Human
Matsuzawa T et al. J Immunol 2012; 189 (2) : 813-8 IFN-γ elicits macrophage autophagy via the p38 MAPK signaling pathway.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169