Isl1 antibody

Cat. No. GTX128201

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Wm
Reactivity	Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all zebrafish Isl1 products

View product citations for antibody GTX128201 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
IHC-Wm	1:100-1:500

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Bovine (100%)| Chicken (99%)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Isl1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2868422

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ISL LIM homeobox 1 , Isl-1 , islet-1

Cellular Localization

Nucleus

Background

Binds to one of the cis-acting domain of the insulin gene enhancer. May be involved in subtype specialization of primary motoneurons.

Database

Gene ID: 30147 isl1
UniProt: P53405 isl1

Research Area

- Zebrafish

DATA IMAGES

GTX128201 IHC-Wm Image

Isl1 antibody detects Isl1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Isl1 antibody (GTX128201) dilution: 1:50.

GTX128201 IHC-Wm Image

Isl1 antibody detects Isl1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Isl1 stained by Isl1 antibody (GTX128201) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC