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Islet 1 antibody

Orthogonal
Anti-Islet 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102807
Anti-Islet 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102807
Anti-Islet 1 antibody used in Western Blot (WB). GTX102807
Anti-Islet 1 antibody used in Western Blot (WB). GTX102807
Anti-Islet 1 antibody used in Western Blot (WB). GTX102807
Anti-Islet 1 antibody used in Western Blot (WB). GTX102807
Anti-Islet 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102807

Cat. No. GTX102807

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Islet 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

PC-12

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Islet 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179180

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ISL LIM homeobox 1 , ISLET1 , Isl-1

Cellular Localization

Nucleus

Background

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Islet 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102807

GTX102807 IHC-Fr Image

Islet 1 antibody detects Islet 1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Islet 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102807

GTX102807 ICC/IF Image

Islet 1 antibody detects Islet 1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Islet 1 protein stained by Islet 1 antibody (GTX102807) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Islet 1 antibody used in Western Blot (WB). GTX102807

GTX102807 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Islet 1 antibody used in Western Blot (WB). GTX102807

GTX102807 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Islet 1 antibody used in Western Blot (WB). GTX102807

GTX102807 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Islet 1 antibody used in Western Blot (WB). GTX102807

GTX102807 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody (GTX102807) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Islet 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102807

GTX102807 IHC-P Image

Islet 1 antibody detects Islet 1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Islet 1 antibody (GTX102807) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sun K et al. iScience 2023; 26 (11) : 108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Lingling Chen et al. J Nanobiotechnology 2022; 20 (1) : 210 Conducting molybdenum sulfide/graphene oxide/polyvinyl alcohol nanocomposite hydrogel for repairing spinal cord injury.
Application : WB, IHC-P
Reactivity : Mouse
Li J et al. Nucleic Acids Res 2021; 49 (4) : 2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.
Application : IHC-Wm
Reactivity : Zebrafish
Kudoh S et al. Histochem Cell Biol 2020; Significance of achaete-scute complex homologue 1 (ASCL1) in pulmonary neuroendocrine carcinomas; RNA sequence analyses using small cell lung cancer cells and Ascl1-induced pulmonary neuroendocrine carcinoma cells.
Application : IHC-P
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169