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JAK1 (phospho Tyr1022) antibody

Anti-JAK1 (phospho Tyr1022) antibody used in Western Blot (WB). GTX50207
Anti-JAK1 (phospho Tyr1022) antibody used in IHC (Paraffin sections) (IHC-P). GTX50207

Cat. No. GTX50207

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all JAK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 1022 (K-E-Y(p)-Y-T) derived from human JAK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Janus kinase 1 , JAK1A , JAK1B , JTK3

Background

This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-JAK1 (phospho Tyr1022) antibody used in Western Blot (WB). GTX50207

GTX50207 WB Image

WB analysis of extracts from HT-29 cells untreated or treated with UV using GTX50207 JAK1 (phospho Tyr1022) antibody.

Anti-JAK1 (phospho Tyr1022) antibody used in IHC (Paraffin sections) (IHC-P). GTX50207

GTX50207 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50207 JAK1 (phospho Tyr1022) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Wu R et al. J Immunother Cancer 2020; 8 (2) SOX2 promotes resistance of melanoma with PD-L1 high expression to T-cell-mediated cytotoxicity that can be reversed by SAHA.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399