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JAK2 antibody [AT53B7]

Anti-JAK2 antibody [AT53B7] used in Flow cytometry (FACS). GTX57670
Anti-JAK2 antibody [AT53B7] used in Western Blot (WB). GTX57670
Anti-JAK2 antibody [AT53B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57670

Cat. No. GTX57670

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT53B7

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all JAK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT53B7 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human JAK4 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Janus kinase 2 , JAK2 , JTK10 , THCYT3

Cellular Localization

Endomembrane system,Cytoplasm,Nucleus

Background

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-JAK2 antibody [AT53B7] used in Flow cytometry (FACS). GTX57670

GTX57670 FACS Image

FACS analysis of HeLa cells using GTX57670 JAK2 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-JAK2 antibody [AT53B7] used in Western Blot (WB). GTX57670

GTX57670 WB Image

WB analysis of various samples using GTX57670 JAK2 antibody.
Lane 1 : K562 whole cell lysate
Lane 2 : TF-1 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : THP-1 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-JAK2 antibody [AT53B7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57670

GTX57670 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57670 JAK2 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399