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JAM-A antibody

Anti-JAM-A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53992
Anti-JAM-A antibody used in IHC (Paraffin sections) (IHC-P). GTX53992
Anti-JAM-A antibody used in Western Blot (WB). GTX53992
Anti-JAM-A antibody used in IHC (Paraffin sections) (IHC-P). GTX53992

Cat. No. GTX53992

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all JAM-A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-238 of human F11R (NP_058642.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F11 receptor , CD321 , JAM , JAM1 , JAMA , JCAM , KAT , PAM-1

Cellular Localization

Cell membrane

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-JAM-A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53992

GTX53992 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX53992 JAM-A antibody.
Blue : DAPI

Anti-JAM-A antibody used in IHC (Paraffin sections) (IHC-P). GTX53992

GTX53992 IHC-P Image

IHC-P analysis of human placenta tissue using GTX53992 JAM-A antibody.
Dilution : 1:100

Anti-JAM-A antibody used in Western Blot (WB). GTX53992

GTX53992 WB Image

WB analysis of various sample lysates using GTX53992 JAM-A antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-JAM-A antibody used in IHC (Paraffin sections) (IHC-P). GTX53992

GTX53992 IHC-P Image

IHC-P analysis of rat brain tissue using GTX53992 JAM-A antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399