APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Liver Tissue Lysate
Product Note
This antibody will not cross-react with JMJD1A or JMJD1B.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
JMJD1C antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human JMJD1C.The immunogen is located within amino acids 380 - 430 of JMJD1C.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
jumonji domain containing 1C , KDM3C , TRIP-8 , TRIP8
Cellular Localization
Nucleus
Background
The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist. This antibody will not cross-react with JMJD1A or JMJD1B.
Database
Research Area
DATA IMAGES
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GTX85014 WB Image
WB analysis of human liver tissue lysate using GTX85014 JMJD1C antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX85014 IHC-P Image
IHC-P analysis of rat liver tissue using GTX85014 JMJD1C antibody.
Working concentration : 2.5 μg/ml
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GTX85014 IHC-P Image
IHC-P analysis of rat liver tissue using GTX85014 JMJD1C antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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