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JNK1 antibody [1E5]

Anti-JNK1 antibody [1E5] used in Western Blot (WB). GTX60600
Anti-JNK1 antibody [1E5] used in Western Blot (WB). GTX60600
Anti-JNK1 antibody [1E5] used in ELISA (ELISA). GTX60600
Anti-JNK1 antibody [1E5] used in Flow cytometry (FACS). GTX60600

Cat. No. GTX60600

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E5

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MAPK8 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 8 , JNK , JNK-46 , JNK1 , JNK1A2 , JNK21B1/2 , PRKM8 , SAPK1 , SAPK1c

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-JNK1 antibody [1E5] used in Western Blot (WB). GTX60600

GTX60600 WB Image

WB analysis of human JNK1 (AA: 227-380) recombinant protein using GTX60600 JNK1 antibody [1E5].

Anti-JNK1 antibody [1E5] used in Western Blot (WB). GTX60600

GTX60600 WB Image

WB analysis of A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate using GTX60600 JNK1 antibody [1E5].

Anti-JNK1 antibody [1E5] used in ELISA (ELISA). GTX60600

GTX60600 ELISA Image

ELISA analysis of antigen using GTX60600 JNK1 antibody [1E5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-JNK1 antibody [1E5] used in Flow cytometry (FACS). GTX60600

GTX60600 FACS Image

FACS analysis of HeLa cells using GTX60600 JNK1 antibody [1E5].
Green : JNK1
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409