APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KIFAP3 (NP_055785.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
kinesin associated protein 3 , FLA3 , KAP-1 , KAP-3 , KAP3 , SMAP , Smg-GDS , dJ190I16.1
Background
The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
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GTX64954 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX64954 KAP3 antibody.
Blue : DAPI
Dilution : 1:100
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GTX64954 WB Image
WB analysis of various sample lysates using GTX64954 KAP3 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX64954 ICC/IF Image
ICC/IF analysis of H9C2 cells using GTX64954 KAP3 antibody.
Blue : DAPI
Dilution : 1:100
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GTX64954 ICC/IF Image
ICC/IF analysis of L929 cells using GTX64954 KAP3 antibody.
Blue : DAPI
Dilution : 1:100
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GTX64954 IP Image
IP analysis of HeLa cell lysate using GTX64954 KAP3 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
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REFERENCE
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REVIEW
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