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KCNMB1 antibody

KO/KD
Anti-KCNMB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23587
Anti-KCNMB1 antibody used in Western Blot (WB). GTX23587
Anti-KCNMB1 antibody used in Western Blot (WB). GTX23587
Anti-KCNMB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23587

Cat. No. GTX23587

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Dog
Package
100 μg ($399)
See all KCNMB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2.0 μg/ml
ICC/IF 1:100-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

This antibody detects large conductance, calcium-activated potassium channel beta (Maxi K+ Beta). The molecular weight detected varies due to glycosylation, multimerization and binding to actin.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic Peptide: L(90) Y H T E D T R D Q N Q Q C(103)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

potassium calcium-activated channel subfamily M regulatory beta subunit 1 , BKbeta , BKbeta1 , k(VCA)beta-1 , slo-beta , slo-beta-1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

modulatory subunit of the MaxiK large conductance potassium channel; voltage and calcium-sensitive potassium channel [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-KCNMB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23587

GTX23587 ICC/IF Image

KO/KD

ICC/IF analysis of MOVAS cells transfected with (+) and without (-) Maxi K+ Beta (KCNMB1) siRNA using GTX23587 KCNMB1 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes
Dilution : 1:100 in staining buffer for 1 hour at room temperature

Anti-KCNMB1 antibody used in Western Blot (WB). GTX23587

GTX23587 WB Image

KO/KD

WB analysis of MOVAS whole cell lysates with and without Maxi K+ Beta (KCNMB1) siRNA using GTX23587 KCNMB1 antibody.
Dilution : 1 μg/ml
Loading : 20 μg

Anti-KCNMB1 antibody used in Western Blot (WB). GTX23587

GTX23587 WB Image

WB analysis of 20 ug of Movas whole cell lysates using GTX23587 KCNMB1 antibody.
Dilution : 1 μg/ml

Anti-KCNMB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23587

GTX23587 ICC/IF Image

ICC/IF analysis of Movas cells using GTX23587 KCNMB1 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes
Dilution : 1:100 in staining buffer for 1 hour at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399