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KCTD5 antibody [3C8]

Overexpression
Anti-KCTD5 antibody [3C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84281
Anti-KCTD5 antibody [3C8] used in Western Blot (WB). GTX84281
Anti-KCTD5 antibody [3C8] used in IHC (Paraffin sections) (IHC-P). GTX84281
Anti-KCTD5 antibody [3C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84281
Anti-KCTD5 antibody [3C8] used in IHC (Paraffin sections) (IHC-P). GTX84281
Anti-KCTD5 antibody [3C8] used in Flow cytometry (FACS). GTX84281
Anti-KCTD5 antibody [3C8] used in IHC (Paraffin sections) (IHC-P). GTX84281
Anti-KCTD5 antibody [3C8] used in Western Blot (WB). GTX84281

Cat. No. GTX84281

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3C8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($539)
See all KCTD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
ICC/IF Assay dependent
IHC-P 1:100-400
FACS Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human KCTD5 (NP_061865) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

potassium channel tetramerization domain containing 5

Cellular Localization

Cytoplasm, cytosol,Nucleus

Background

Its interaction with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complex. Does not affect the function of Kv channel Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2 and Kv3.4/KCNC4.

Database

Research Area

DATA IMAGES

Anti-KCTD5 antibody [3C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84281

GTX84281 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with KCTD5 plasmid using GTX84281 KCTD5 antibody [3C8].

Anti-KCTD5 antibody [3C8] used in Western Blot (WB). GTX84281

GTX84281 WB Image

WB analysis of various cell lines using GTX84281 KCTD5 antibody [3C8].
Loading : 35 ug per lane
Dilution : 1:200

Anti-KCTD5 antibody [3C8] used in IHC (Paraffin sections) (IHC-P). GTX84281

GTX84281 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84281 KCTD5 antibody [3C8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-KCTD5 antibody [3C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84281

GTX84281 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84281 KCTD5 antibody [3C8].

Anti-KCTD5 antibody [3C8] used in IHC (Paraffin sections) (IHC-P). GTX84281

GTX84281 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84281 KCTD5 antibody [3C8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-KCTD5 antibody [3C8] used in Flow cytometry (FACS). GTX84281

GTX84281 FACS Image

FACS analysis of HEK293T cells transfected with either KCTD5 plasmid(Red) or empty vector control plasmid(Blue) using GTX84281 KCTD5 antibody [3C8].

Anti-KCTD5 antibody [3C8] used in IHC (Paraffin sections) (IHC-P). GTX84281

GTX84281 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84281 KCTD5 antibody [3C8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-KCTD5 antibody [3C8] used in Western Blot (WB). GTX84281

GTX84281 WB Image

Overexpression

WB analysis of HEK293T cells transfected with KCTD5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84281 KCTD5 antibody [3C8].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539