KDM4C antibody [5B9]

Cat. No. GTX84280

Host	Mouse
Clonality	Monoclonal
Clone Name	5B9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($519)

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Datasheet
Document

View product citations for antibody GTX84280 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

120 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human KDM4C expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 4C , GASC1 , JHDM3C , JMJD2C , TDRD14C

Cellular Localization

Nucleus

Background

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

Database

Gene ID: 23081 KDM4C
UniProt: Q9H3R0 KDM4C

Research Area

DATA IMAGES

GTX84280 IHC-P Image

IHC-P analysis of colon tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 WB Image

WB analysis of various cell lines using GTX84280 KDM4C antibody [5B9].
Loading : 35 ug per lane
Dilution : 1:200

GTX84280 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 IHC-P Image

IHC-P analysis of ovary tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 IHC-P Image

IHC-P analysis of prostate tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 WB Image

WB analysis of HEK293T cells transfected with KDM4C plasmid (Right) or empty vector (Left) for 48 hrs using GTX84280 KDM4C antibody [5B9].
Loading : 5 ug per lane

GTX84280 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with KDM4C plasmid using GTX84280 KDM4C antibody [5B9].

GTX84280 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84280 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84280 KDM4C antibody [5B9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

KDM4C antibody [5B9]

Cat. No. GTX84280

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84280 IHC-P Image

GTX84280 WB Image

GTX84280 IHC-P Image

GTX84280 IHC-P Image

GTX84280 IHC-P Image

GTX84280 IHC-P Image

GTX84280 IHC-P Image

GTX84280 WB Image

GTX84280 IHC-P Image

GTX84280 ICC/IF Image

GTX84280 IHC-P Image

GTX84280 IHC-P Image

GTX84280 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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