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KEAP1 antibody

KO/KD
Anti-KEAP1 antibody used in Western Blot (WB). GTX31864
Anti-KEAP1 antibody used in Western Blot (WB). GTX31864
Anti-KEAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31864
Anti-KEAP1 antibody used in Western Blot (WB). GTX31864

Cat. No. GTX31864

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all KEAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Human Lung Tissue Lysate(GTX27917) , Human Lung Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1.The immunogen is located within the last 50 amino acids of KEAP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887631

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kelch like ECH associated protein 1 , INrf2 , KLHL19

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-KEAP1 antibody used in Western Blot (WB). GTX31864

GTX31864 WB Image

WB analysis of human lung tissue lysate using GTX31864 KEAP1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-KEAP1 antibody used in Western Blot (WB). GTX31864

GTX31864 WB Image

WB analysis of various sample lysates using GTX31864 KEAP1 antibody.
Loading : 15μg of lysates per lane
Dilution : 2 μg/ml

Anti-KEAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31864

GTX31864 IHC-P Image

IHC-P analysis of human lung tissue using GTX31864 KEAP1 antibody.
Working concentration : 20 μg/ml

Anti-KEAP1 antibody used in Western Blot (WB). GTX31864

GTX31864 WB Image

KO/KD

WB analysis of HeLa cell or KEAP1 KO cell lysates using GTX30005 KEAP1 antibody.
Dilution : 2μg/mL
Loading : 10μg

REFERENCE

Application Reference

Chen HY et al. Chem Biol Interact 2020; 331 : 109249 TGF-β1 signaling protects retinal ganglion cells from oxidative stress via modulation of the HO-1/Nrf2 pathway.
Application : WB
Reactivity : Mouse
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399