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KLC1 antibody [N2C2], Internal

KO/KD Orthogonal
Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510
Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510
Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510
Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510

Cat. No. GTX114510

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all KLC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Drosophila, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620152

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin light chain 1 , KLC , KNS2 , KNS2A

Background

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510

GTX114510 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX114510 diluted at 1:1000

Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510

GTX114510 WB Image

KO/KD Orthogonal

The data was published in the journal PLoS Pathog in 2015. PMID: 25760349

Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510

GTX114510 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31578382

Anti-KLC1 antibody [N2C2], Internal used in Western Blot (WB). GTX114510

GTX114510 WB Image

The data was published in the journal PLoS Pathog in 2015. PMID: 25760349

REFERENCE

Application Reference

Holt I et al. Sci Rep 2019; 9 (1) : 14202 Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle.
Application : WB
Reactivity : Human
Carpentier DC et al. PLoS Pathog 2015; 11 (3) : e1004723 Vaccinia virus protein complex F12/E2 interacts with kinesin light chain isoform 2 to engage the kinesin-1 motor complex.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169