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KLF4 antibody [AT4E6]

Anti-KLF4 antibody [AT4E6] used in Western Blot (WB). GTX57568
Anti-KLF4 antibody [AT4E6] used in Flow cytometry (FACS). GTX57568
Anti-KLF4 antibody [AT4E6] used in Flow cytometry (FACS). GTX57568

Cat. No. GTX57568

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT4E6

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399)
See all KLF4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
FACS Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT4E6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human kLF6 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Kruppel like factor 4 , EZF , GKLF , KLF4

Cellular Localization

Nucleus

Background

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-KLF4 antibody [AT4E6] used in Western Blot (WB). GTX57568

GTX57568 WB Image

WB analysis of various samples using GTX57568 KLF4 antibody.
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Loading : 40 μg
Dilution : 1:3000

Anti-KLF4 antibody [AT4E6] used in Flow cytometry (FACS). GTX57568

GTX57568 FACS Image

FACS analysis of HeLa cells using GTX57568 KLF4 antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg

Anti-KLF4 antibody [AT4E6] used in Flow cytometry (FACS). GTX57568

GTX57568 FACS Image

FACS analysis of A549 cells using GTX57568 KLF4 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399