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KLF9 antibody

Orthogonal
Anti-KLF9 antibody used in Immunoprecipitation (IP). GTX129316
Anti-KLF9 antibody used in Western Blot (WB). GTX129316
Anti-KLF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129316
Anti-KLF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129316
Anti-KLF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129316

Cat. No. GTX129316

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all KLF9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HeLa nuclear

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human KLF9 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885966

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Kruppel like factor 9 , BTEB , BTEB1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor that binds to GC box elements located in the promoter. Binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KLF9 antibody used in Immunoprecipitation (IP). GTX129316

GTX129316 IP Image

Immunoprecipitation of KLF9 protein from HeLa whole cell extracts using 5 μg of KLF9 antibody (GTX129316).
Western blot analysis was performed using KLF9 antibody (GTX129316).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-KLF9 antibody used in Western Blot (WB). GTX129316

GTX129316 WB Image

Orthogonal

KLF9 antibody detects KLF9 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
12 % SDS-PAGE
KLF9 antibody (GTX129316) dilution: 1:1000

Anti-KLF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129316

GTX129316 IHC-P Image

KLF9 antibody detects KLF9 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF9 stained by KLF9 antibody (GTX129316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129316

GTX129316 IHC-P Image

KLF9 antibody detects KLF9 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
KLF9 stained by KLF9 antibody (GTX129316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX129316

GTX129316 IHC-P Image

KLF9 antibody detects KLF9 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
KLF9 stained by KLF9 antibody (GTX129316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhang J et al. eNeuro 2023; 10 (11) Electroacupuncture Relieves HuR/KLF9-Mediated Inflammation to Enhance Neurological Repair after Spinal Cord Injury.
Lili LN et al. J Invest Dermatol 2019; 139 (11) : 2281-2291 Transcriptomic Network Interactions in Human Skin Treated with Topical Glucocorticoid Clobetasol Propionate.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169