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KLHL15 antibody

Anti-KLHL15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85275
Anti-KLHL15 antibody used in Western Blot (WB). GTX85275
Anti-KLHL15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85275

Cat. No. GTX85275

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all KLHL15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLHL15 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human KLHL15.The immunogen is located within the last 50 amino acids of KLHL15.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kelch like family member 15 , HEL-S-305

Cellular Localization

Nucleus

Background

KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like proteins have been implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL15 mRNA is widely expressed in many tissues and is thought to be involved in protein ubiquination and cytoskeleton organization.

Database

Research Area

DATA IMAGES

Anti-KLHL15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85275

GTX85275 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85275 KLHL15 antibody.
Working concentration : 20 μg/ml

Anti-KLHL15 antibody used in Western Blot (WB). GTX85275

GTX85275 WB Image

WB analysis of HeLa cell lysate using GTX85275 KLHL15 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-KLHL15 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85275

GTX85275 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX85275 KLHL15 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409