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L3MBTL1 antibody

Anti-L3MBTL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103793
Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793
Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793

Cat. No. GTX103793

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Caenorhabditis elegans
Package
100 μl ($399),
25 μl ($169)
See all L3MBTL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HeLaS3 , human L3MBTL-transfected 293T cells

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human L3MBTL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950656

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

L3MBTL histone methyl-lysine binding protein 1 , H-L(3)MBT , L3MBTL , ZC2HC3 , dJ138B7.3

Cellular Localization

Nucleus

Background

This gene encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-L3MBTL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103793

GTX103793 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using L3MBTL(GTX103793) antibody at 1:200 dilution.

Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793

GTX103793 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX103793 diluted at 1:1000

Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793

GTX103793 WB Image

L3MBTL antibody detects L3MBTL protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human L3MBTL-transfected 293T cells
7.5 % SDS-PAGE
L3MBTL antibody (GTX103793) dilution: 1:5000

REFERENCE

Application Reference

Lu J et al. Nat Neurosci 2019; 22 (6) : 875-886 L3MBTL1 regulates ALS/FTD-associated proteotoxicity and quality control.
Application : IHC-Fr
Reactivity : Caenorhabditis elegans
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169