GeneTex

  • Country / Location Selection

United States (US)

LDB2 antibody

Anti-LDB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX23627
Anti-LDB2 antibody used in Western Blot (WB). GTX23627
Anti-LDB2 antibody used in Western Blot (WB). GTX23627
Anti-LDB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX23627

Cat. No. GTX23627

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($589)
See all LDB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 5 μg/mL
ELISA 1:5000-1:20000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM domain binding 2 , CLIM1 , LDB-2 , LDB1

Cellular Localization

Nucleus

Background

It is suggested that LDB1 and LDB2 act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB 2 is a close homologue of LDB 1.

Database

DATA IMAGES

Anti-LDB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX23627

GTX23627 IHC-P Image

GeneTex's Affinity Purified anti-LDB2 (Clim1) antibody was used at a 5 μg/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.

Anti-LDB2 antibody used in Western Blot (WB). GTX23627

GTX23627 WB Image

Western blot using GeneTex's Affinity Purified anti-LDB2 antibody shows detection of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Anti-LDB2 antibody used in Western Blot (WB). GTX23627

GTX23627 WB Image

WB analysis of various samples using GTX23627 LDB2 antibody.
Lane 1 : Human kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Loading : 18 μg
Dilution : 1:500

Anti-LDB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX23627

GTX23627 IHC-P Image

IHC-P analysis of human brain cortex tissue using GTX23627 LDB2 antibody.
Dilution : 5 μg/mL

REFERENCE

There are currently no references for LDB2 antibody (GTX23627). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for LDB2 antibody (GTX23627). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 589