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LDH-B antibody

KO/KD Orthogonal
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747
Anti-LDH-B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747

Cat. No. GTX101747

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all LDH-B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDH-B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037332

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lactate dehydrogenase B , HEL-S-281 , LDH-B , LDH-H , LDHBD , TRG-5

Cellular Localization

Cytoplasm

Background

This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

KO/KD

Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747

GTX101747 IHC-P Image

LDH-B antibody detects LDH-B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
LDH-B stained by LDH-B antibody (GTX101747) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747

GTX101747 IHC-P Image

LDH-B antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
LDH-B antibody (GTX101747) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LDH-B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101747

GTX101747 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using LDH-B(GTX101747) antibody at 1:50 dilution.

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Orthogonal

The data was published in the journal BMC Genomics in 2015. PMID: 25652794

REFERENCE

Application Reference

Goto T et al. Exp Hematol 2019; (Epub) ATP produced by anaerobic glycolysis is essential for enucleation of human erythroblasts.
Application : WB
Reactivity : Human
Chen CN et al. Am J Physiol Regul Integr Comp Physiol 2017; : ajpregu.00337.2016 Diet-induced obesity accelerates blood lactate accumulation of rats in response to incremental exercise to maximum.
Application : WB
Reactivity : Rat
Lin LL et al. BMC Genomics 2015; 16 : 41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.
Application : WB
Reactivity : Human
Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

LDH-B antibody Cat. No. GTX101747

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Blocking : 5%milk in TBST
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169