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LEMD3 antibody [3H1]

Anti-LEMD3 antibody [3H1] used in Flow cytometry (FACS). GTX84221
Anti-LEMD3 antibody [3H1] used in Immunoprecipitation (IP). GTX84221
Anti-LEMD3 antibody [3H1] used in Western Blot (WB). GTX84221
Anti-LEMD3 antibody [3H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84221
Anti-LEMD3 antibody [3H1] used in Flow cytometry (FACS). GTX84221

Cat. No. GTX84221

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3H1

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all LEMD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
FACS 1:100
IP 2-4ug/mg
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LEM domain containing 3 , MAN1

Cellular Localization

Nucleus inner membrane

Background

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.

Database

Research Area

DATA IMAGES

Anti-LEMD3 antibody [3H1] used in Flow cytometry (FACS). GTX84221

GTX84221 FACS Image

FACS analysis of HeLa cells using GTX84221 LEMD3 antibody [3H1].
Red : Primary antibody
Blue : Negative control antibody

Anti-LEMD3 antibody [3H1] used in Immunoprecipitation (IP). GTX84221

GTX84221 IP Image

IP analysis of DDDDK tagged LEMD3 overexpressed HEK293T lysate using GTX84221 LEMD3 antibody [3H1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-LEMD3 antibody [3H1] used in Western Blot (WB). GTX84221

GTX84221 WB Image

WB analysis of various cell lines using GTX84221 LEMD3 antibody [3H1].
Loading : 35 ug per lane

Anti-LEMD3 antibody [3H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84221

GTX84221 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with LEMD3 plasmid using GTX84221 LEMD3 antibody [3H1].

Anti-LEMD3 antibody [3H1] used in Flow cytometry (FACS). GTX84221

GTX84221 FACS Image

FACS analysis of Jurkat cells using GTX84221 LEMD3 antibody [3H1].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519