APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human LGALS3BP
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
galectin 3 binding protein , 90K , BTBD17B , CyCAP , M2BP , MAC-2-BP , TANGO10B , gp90
Cellular Localization
Secreted,Secreted, extracellular space, extracellular matrix
Background
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146](/templates/default/js/lightbox/images/loading.gif "Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146")
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GTX638146 ICC/IF Image
LGALS3BP antibody [HL2168] detects LGALS3BP protein at endoplasmic reticulum and cell junction by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LGALS3BP stained by LGALS3BP antibody [HL2168] (GTX638146) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638146 ICC/IF Image
LGALS3BP antibody [HL2168] detects LGALS3BP protein at cytoplasmic vesicle by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: LGALS3BP stained by LGALS3BP antibody [HL2168] (GTX638146) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638146 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGALS3BP antibody [HL2168] (GTX638146) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638146 IHC-P Image
LGALS3BP antibody [HL2168] detects secreted LGALS3BP protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse adrenal gland.LGALS3BP stained by LGALS3BP antibody [HL2168] (GTX638146) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638146 WB Image
Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGALS3BP antibody [HL2168] (GTX638146) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for LGALS3BP antibody [HL2168] (GTX638146). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for LGALS3BP antibody [HL2168] (GTX638146). Be the first to share your experience with this product.
![Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146](/upload/website/prouct_img/small/GTX638146/GTX638146_44998_20230526_ICC_IF_23060622_592.webp "Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146](/upload/website/prouct_img/small/GTX638146/GTX638146_44998_20230512_ICC_IF_23060622_282.webp "Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146](/upload/website/prouct_img/small/GTX638146/GTX638146_44998_20230331_WB_TPM_watermark_23041023_629.webp "Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in IHC (Paraffin sections) (IHC-P). GTX638146](/upload/website/prouct_img/small/GTX638146/GTX638146_T-44928_20230217_IHC-P_M_23021401_943.webp "Anti-LGALS3BP antibody [HL2168] used in IHC (Paraffin sections) (IHC-P). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146](/upload/website/prouct_img/small/GTX638146/GTX638146_T-44928_20230217_WB_treatment_Tunicamycin_23022022_225.webp "Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146](/upload/website/prouct_img/normal/GTX638146/GTX638146_44998_20230526_ICC_IF_23060622_592.webp "Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146](/upload/website/prouct_img/normal/GTX638146/GTX638146_44998_20230512_ICC_IF_23060622_282.webp "Anti-LGALS3BP antibody [HL2168] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146](/upload/website/prouct_img/normal/GTX638146/GTX638146_44998_20230331_WB_TPM_watermark_23041023_629.webp "Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in IHC (Paraffin sections) (IHC-P). GTX638146](/upload/website/prouct_img/normal/GTX638146/GTX638146_T-44928_20230217_IHC-P_M_23021401_943.webp "Anti-LGALS3BP antibody [HL2168] used in IHC (Paraffin sections) (IHC-P). GTX638146")
![Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146](/upload/website/prouct_img/normal/GTX638146/GTX638146_T-44928_20230217_WB_treatment_Tunicamycin_23022022_225.webp "Anti-LGALS3BP antibody [HL2168] used in Western Blot (WB). GTX638146")