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LIMS1 antibody [5G7]

Anti-LIMS1 antibody [5G7] used in Western Blot (WB). GTX60472
Anti-LIMS1 antibody [5G7] used in Flow cytometry (FACS). GTX60472
Anti-LIMS1 antibody [5G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60472
Anti-LIMS1 antibody [5G7] used in ELISA (ELISA). GTX60472
Anti-LIMS1 antibody [5G7] used in Western Blot (WB). GTX60472

Cat. No. GTX60472

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G7

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all LIMS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human LIMS1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM zinc finger domain containing 1 , PINCH , PINCH-1 , PINCH1

Background

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-LIMS1 antibody [5G7] used in Western Blot (WB). GTX60472

GTX60472 WB Image

WB analysis of human LIMS1 (AA: 87-249) recombinant protein using GTX60472 LIMS1 antibody [5G7].

Anti-LIMS1 antibody [5G7] used in Flow cytometry (FACS). GTX60472

GTX60472 FACS Image

FACS analysis of HeLa cells using GTX60472 LIMS1 antibody [5G7].
Blue : LIMS1
Red : negative control

Anti-LIMS1 antibody [5G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60472

GTX60472 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60472 LIMS1 antibody [5G7].
Green : LIMS1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-LIMS1 antibody [5G7] used in ELISA (ELISA). GTX60472

GTX60472 ELISA Image

ELISA analysis of antigen using GTX60472 LIMS1 antibody [5G7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-LIMS1 antibody [5G7] used in Western Blot (WB). GTX60472

GTX60472 WB Image

WB analysis of A549 (1), Jurkat (2), and HeLa (3) cell lysate using GTX60472 LIMS1 antibody [5G7].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409