LIN7A antibody [GT377]

Cat. No. GTX631106

Host	Mouse
Clonality	Monoclonal
Clone Name	GT377
Isotype	IgG2b
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX631106 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888239

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lin-7 homolog A, crumbs cell polarity complex component , LIN-7A , LIN7 , MALS-1 , MALS1 , TIP-33 , VELI1

Cellular Localization

Cell membrane; Peripheral membrane protein , Basolateral cell membrane; Peripheral membrane protein , Cell junction , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density; Peripheral membrane protein

Background

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.

Database

Gene ID: 8825 LIN7A
UniProt: O14910 LIN7A

Research Area

DATA IMAGES

GTX631106 ICC/IF Image

LIN7A antibody [GT377] detects LIN7A protein at junction by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LIN7A protein stained by LIN7A antibody [GT377] (GTX631106) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX631106 WB Image

LIN7A antibody [GT377] detects LIN7A protein by western blot analysis.
A. 30 μg MCF-7 whole cell extract
B. 30 μgMDA-MB-231 whole cell extract
12 % SDS-PAGE
LIN7A antibody [GT377] (GTX631106) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations