GeneTex

  • Country / Location Selection

United States (US)

LIN7A antibody [N1C3]

Anti-LIN7A antibody [N1C3] used in Western Blot (WB). GTX117114
Anti-LIN7A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117114
Anti-LIN7A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117114
Anti-LIN7A antibody [N1C3] used in Western Blot (WB). GTX117114
Anti-LIN7A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117114
Anti-LIN7A antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX117114

Cat. No. GTX117114

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all LIN7A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731933

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lin-7 homolog A, crumbs cell polarity complex component , LIN-7A , LIN7 , MALS-1 , MALS1 , TIP-33 , VELI1

Cellular Localization

Cell membrane; Peripheral membrane protein , Basolateral cell membrane; Peripheral membrane protein , Cell junction , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density; Peripheral membrane protein

Background

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.

Database

Research Area

DATA IMAGES

Anti-LIN7A antibody [N1C3] used in Western Blot (WB). GTX117114

GTX117114 WB Image

Sample (30 μg of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX117114 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIN7A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117114

GTX117114 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LIN7A(GTX117114) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LIN7A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117114

GTX117114 ICC/IF Image

LIN7A antibody [N1C3] detects LIN7A protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LIN7A protein stained by LIN7A antibody [N1C3] (GTX117114) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-LIN7A antibody [N1C3] used in Western Blot (WB). GTX117114

GTX117114 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
12% SDS PAGE
GTX117114 diluted at 1:1000

Anti-LIN7A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117114

GTX117114 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using LIN7A antibody [N1C3] (GTX117114) at 1:300 dilution.

Anti-LIN7A antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX117114

GTX117114 IHC-Wm Image

LIN7A antibody [N1C3] detects Lin7a protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
LIN7A antibody [N1C3] (GTX117114) dilution: 1:200.

REFERENCE

Application Reference

Silvia Jordans et al. Mol Neurobiol 2022; 59 (7) : 3969-3979 Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35.
Application : WB, ICC/IF
Reactivity : Mouse
Yoohwa Chung et al. Biochem Biophys Rep 2020; 24 : 100872 Mint3 is dispensable for pancreatic and kidney functions in mice.
Application : IHC-P
Reactivity : Mouse
Kamijo A et al. Histochem Cell Biol 2019; (Epub) Scaffold protein Lin7 family in membrane skeletal protein complex in mouse seminiferous tubules.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for LIN7A antibody [N1C3] (GTX117114). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169