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LMO4 antibody

Orthogonal
Anti-LMO4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129616
Anti-LMO4 antibody used in Western Blot (WB). GTX129616
Anti-LMO4 antibody used in Western Blot (WB). GTX129616

Cat. No. GTX129616

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC (Free Floating)

Reactivity

 Human, Xenopus laevis
Package
100 μl ($399),
25 μl ($169)
See all LMO4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

human LMO4-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human LMO4 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886055

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM domain only 4

Background

This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LMO4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129616

GTX129616 IHC-P Image

LMO4 antibody detects LMO4 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LMO4 antibody (GTX129616) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LMO4 antibody used in Western Blot (WB). GTX129616

GTX129616 WB Image

LMO4 antibody detects LMO4 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human LMO4-transfected 293T cells
12 % SDS-PAGE
LMO4 antibody (GTX129616) dilution: 1:5000

Anti-LMO4 antibody used in Western Blot (WB). GTX129616

GTX129616 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LMO4 antibody (GTX129616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Jim?nez S et al. Front Neuroanat 2022; 16 : 1039081 Development of subdomains in the medial pallium of Xenopus laevis and Trachemys scripta: Insights into the anamniote-amniote transition.
Application : IHC (Free Floating)
Reactivity : Xenopus laevis
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169