LMX1B antibody

Cat. No. GTX129831

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Fr
Reactivity	Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all LMX1B products

Datasheet
Document

View product citations for antibody GTX129831 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

mouse skeletal muscle

Predict Reactivity

Human, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human LMX1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM homeobox transcription factor 1 beta , LMX1.2 , NPS1

Cellular Localization

Nucleus

Background

This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 4010 LMX1B
UniProt: O60663 LMX1B

Research Area

DATA IMAGES

GTX129831 IHC-Fr Image

LMX1B antibody detects LMX1B protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: LMX1B protein stained by LMX1B antibody (GTX129831) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129831 WB Image

LMX1B antibody detects LMX1B protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with LMX1B antibody (GTX129831) at a dilution of 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty