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LONP1 antibody

Anti-LONP1 antibody used in Flow cytometry (FACS). GTX03806
Anti-LONP1 antibody used in Western Blot (WB). GTX03806
Anti-LONP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03806
Anti-LONP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03806
Anti-LONP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03806
Anti-LONP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03806

Cat. No. GTX03806

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all LONP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/mL
ICC/IF 2μg/mL
IHC-P 0.5-1μg/mL
FACS 1-3μg/1x106 cells
ELISA 0.1-0.5μg/mL
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human LONP1/Lon recombinant protein (Position: R137-A320).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lon peptidase 1, mitochondrial , CODASS , LON , LONP , LonHS , PIM1 , PRSS15 , hLON

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]

Database

Research Area

DATA IMAGES

Anti-LONP1 antibody used in Flow cytometry (FACS). GTX03806

GTX03806 FACS Image

FACS analysis of HL-60 cells using GTX03806 LONP1 antibody.
Antibody amount : 1μg/1x106 cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-LONP1 antibody used in Western Blot (WB). GTX03806

GTX03806 WB Image

WB analysis of various sample lysates using GTX03806 LONP1 antibody.
Lane 1 : K562 cells
Lane 2 : U87-MG cells
Lane 3 : PC-3 cells
Lane 4 : A549 cells
Lane 5 : Mouse liver tissue
Lane 6 : Mouse kidney tissue
Lane 7 : SP20 cells
Dilution : 0.5 μg/mL
Loading : 50 μg per lane

Anti-LONP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03806

GTX03806 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX03806 LONP1 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/mL

Anti-LONP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03806

GTX03806 ICC/IF Image

ICC/IF analysis of A431 cells using GTX03806 LONP1 antibody.
Antigen retrieval : Enzyme antigen retrieval was performed using enzyme antigen retrieval reagent for 15 mins.
Dilution : 2μg/mL

Anti-LONP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03806

GTX03806 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX03806 LONP1 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/mL

Anti-LONP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03806

GTX03806 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX03806 LONP1 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 469