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LY6E antibody [HL1933]

Recombinant antibody KO/KD Orthogonal
Anti-LY6E antibody [HL1933] used in Western Blot (WB). GTX637770
Anti-LY6E antibody [HL1933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637770
Anti-LY6E antibody [HL1933] used in IHC (Paraffin sections) (IHC-P). GTX637770
Anti-LY6E antibody [HL1933] used in Western Blot (WB). GTX637770

Cat. No. GTX637770

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1933

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LY6E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human LY6E

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte antigen 6 family member E , RIG-E , RIGE , SCA-2 , SCA2 , TSA-1

Cellular Localization

Cell membrane

Background

This gene belongs to the human Ly6 gene family and encodes a glycosylphosphatidyl-inositol (GPI)-anchored cell surface protein. The protein plays an important role in T cell physiology, oncogenesis and immunological regulation. The protein is also involved in modulation of viral infection by coronaviruses, SARS-CoV, MERS-CoV and SARS-CoV-2. [provided by RefSeq, Aug 2021]

Database

DATA IMAGES

Anti-LY6E antibody [HL1933] used in Western Blot (WB). GTX637770

GTX637770 WB Image

KO/KD

Non-transfected (–) and transfected (+) A549 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LY6E antibody [HL1933] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637770

GTX637770 ICC/IF Image

LY6E antibody [HL1933] detects LY6E protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LY6E antibody [HL1933] used in IHC (Paraffin sections) (IHC-P). GTX637770

GTX637770 IHC-P Image

LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
LY6E stained by LY6E antibody [HL1933] (GTX637770) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LY6E antibody [HL1933] used in Western Blot (WB). GTX637770

GTX637770 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (GTX637770) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations