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LYAG antibody [C2C3], C-term

KO/KD
Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821
Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821
Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821
Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821

Cat. No. GTX109821

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LYAG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LYAG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950323

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucosidase alpha, acid , LYAG

Cellular Localization

Lysosome

Background

This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821

GTX109821 WB Image

KO/KD

Wild-type (WT) and LYAG knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821

GTX109821 IHC-P Image

LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LYAG antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109821

GTX109821 IHC-P Image

LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LYAG antibody [C2C3], C-term used in Western Blot (WB). GTX109821

GTX109821 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Filomena Napolitano et al. Int J Mol Sci 2021; 22 (7) Rare Variants in Autophagy and Non-Autophagy Genes in Late-Onset Pompe Disease: Suggestions of Their Disease-Modifying Role in Two Italian Families.
Reactivity : Human
Basile I et al. J Control Release 2017; 269 : 15-23 Efficient therapy for refractory Pompe disease by mannose 6-phosphate analogue grafting on acid α-glucosidase.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169