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LYRIC antibody [N2C3]

Anti-LYRIC antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100587
Anti-LYRIC antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100587
Anti-LYRIC antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100587
Anti-LYRIC antibody [N2C3] used in Western Blot (WB). GTX100587
Anti-LYRIC antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100587
Anti-LYRIC antibody [N2C3] used in Western Blot (WB). GTX100587

Cat. No. GTX100587

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all LYRIC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

rat brain

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LYRIC . The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037471

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

metadherin , 3D3 , AEG-1 , AEG1 , LYRIC , LYRIC/3D3

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein , Cell junction , tight junction , Nucleus , nucleolus , Cytoplasm , perinuclear region

Background

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Database

Research Area

DATA IMAGES

Anti-LYRIC antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100587

GTX100587 ICC/IF Image

LYRIC antibody [N2C3] detects LYRIC protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: LYRIC protein stained by LYRIC antibody [N2C3] (GTX100587) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-LYRIC antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100587

GTX100587 IHC-P Image

LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LYRIC antibody [N2C3] (GTX100587) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LYRIC antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100587

GTX100587 IHC-P Image

LYRIC antibody [N2C3] detects LYRIC protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
LYRIC antibody [N2C3] (GTX100587) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LYRIC antibody [N2C3] used in Western Blot (WB). GTX100587

GTX100587 WB Image

LYRIC antibody detects LYRIC protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LYRIC antibody (GTX100587) diluted by 1:1000.
Observed TARGET protein has mutiple bands at 68 kDa, and 80 kDa, possibly due to alternative splicing. Reference: Biochem Biophys Res Commun. 2008 Jun 27;371(2):333-8.

Anti-LYRIC antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX100587

GTX100587 IHC-P Image

LYRIC antibody [N2C3] detects LYRIC protein at cytosol on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
LYRIC antibody [N2C3] (GTX100587) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LYRIC antibody [N2C3] used in Western Blot (WB). GTX100587

GTX100587 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28765561

REFERENCE

Application Reference

Lai YC et al. Sci Rep 2017; 7 (1) : 6975 Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169