GeneTex

  • Country / Location Selection

United States (US)

Lactate Dehydrogenase C antibody

Anti-Lactate Dehydrogenase C antibody used in Western Blot (WB). GTX114525
Anti-Lactate Dehydrogenase C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114525
Anti-Lactate Dehydrogenase C antibody used in IHC (Paraffin sections) (IHC-P). GTX114525

Cat. No. GTX114525

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Lactate Dehydrogenase C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lactate Dehydrogenase C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174283

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lactate dehydrogenase C , CT32 , LDH3 , LDHX

Cellular Localization

Cytoplasm

Background

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lactate Dehydrogenase C antibody used in Western Blot (WB). GTX114525

GTX114525 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX114525 diluted at 1:10000

Anti-Lactate Dehydrogenase C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114525

GTX114525 ICC/IF Image

lactate dehydrogenase C antibody detects lactate dehydrogenase C protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: lactate dehydrogenase C protein stained by lactate dehydrogenase C antibody (GTX114525) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Lactate Dehydrogenase C antibody used in IHC (Paraffin sections) (IHC-P). GTX114525

GTX114525 IHC-P Image

lactate dehydrogenase C antibody detects LDHC protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
lactate dehydrogenase C antibody (GTX114525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Dominguez G et al. Acta Neuropathol Commun 2018; 6 (1) : 25 Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain.
Application : IHC-Fr
Reactivity : Human
Jan CI et al. Int J Biol Sci? 2016; 12 (7) : 786-798 Fenofibrate Suppresses Oral Tumorigenesis via Reprogramming Metabolic Processes: Potential Drug Repurposing for Oral Cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Lactate Dehydrogenase C antibody (GTX114525). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169