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Laminin alpha 5 antibody

Anti-Laminin alpha 5 antibody used in Western Blot (WB). GTX55867
Anti-Laminin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55867

Cat. No. GTX55867

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Laminin alpha 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

400 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Laminin alpha 5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887940

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

laminin subunit alpha 5

Background

This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-Laminin alpha 5 antibody used in Western Blot (WB). GTX55867

GTX55867 WB Image

WB analysis of JAR (A), A431 (B), RAW264.7 (C) whole cell lysates using GTX55867 Laminin alpha 5 antibody.

Anti-Laminin alpha 5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55867

GTX55867 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55867 Laminin alpha 5 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Sasamoto Y et al. Cell Rep 2022; 40 (6) : 111166 Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.
Application : WB
Reactivity : Human
Uchio-Yamada K et al. Am J Physiol Renal Physiol 2020; 318 (6) : F1520-F1530 Tensin2 is important for podocyte-glomerular basement membrane interaction and integrity of the glomerular filtration barrier.
Application : IHC-Fr
Reactivity : Mouse
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399