Laminin beta 3 antibody [C1C2], Internal

Name: Laminin beta 3 antibody [C1C2], Internal
Brand: GeneTex
SKU: GTX103736
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Laminin beta 3 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103736

Anti-Laminin beta 3 antibody [C1C2], Internal used in Western Blot (WB). GTX103736

Anti-Laminin beta 3 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103736

Cat. No. GTX103736

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Laminin beta 3 products

View product citations for antibody GTX103736 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

NIH-3T3 , A431

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Laminin beta 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037334

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

laminin subunit beta 3 , AI1A , BM600-125KDA , LAM5 , LAMNB1

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane

Background

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3914 LAMB3
UniProt: Q13751 LAMB3

Research Area

DATA IMAGES

GTX103736 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using Laminin beta 3(GTX103736) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103736 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX103736 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103736 ICC/IF Image

Laminin beta 3 antibody [C1C2], Internal detects Laminin beta 3 protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Laminin beta 3 stained by Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX103736 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Laminin beta 3 antibody [C1C2], Internal (GTX103736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Uehara N et al. Lab Invest 2017; (Epub) Osteoblast-derived Laminin-332 is a novel negative regulator of osteoclastogenesis in bone microenvironments.

Application : IHC-P

Reactivity : Mouse

Submit a Reference

REVIEW