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Lck (phospho Tyr394) antibody

Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX38561

Cat. No. GTX38561

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all Lck products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Lck around the phosphorylation site of tyrosine 393 (N-E-Yp-T-A).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11174674

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IMD22,LCK,LCK protooncogene, Src family tyrosine kinase,LSK,YT16,p56lck,pp58lck,Lck,LCK proto-oncogene, Src family tyrosine kinase

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-Lck (phospho Tyr394) antibody used in Western Blot (WB). GTX38561

GTX38561 WB Image

WB analysis of MOLT cell lysate using GTX38561 Lck (phospho Tyr394) antibody.

REFERENCE

Application Reference

Davenport AJ et al. Proc Natl Acad Sci U S A 2018; (Epub) Chimeric antigen receptor T cells form nonclassical and potent immune synapses driving rapid cytotoxicity.
Application : WB
Reactivity : Mouse
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Package List Price ($)
$ 399