GeneTex

  • Country / Location Selection

United States (US)

Legionella pneumophila antibody

Anti-Legionella pneumophila antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64135
Anti-Legionella pneumophila antibody used in ELISA (ELISA). GTX64135

Cat. No. GTX64135

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, ELISA, Agglutination, IHC

Reactivity

 Legionella pneumophila
Package
200 μl ($399)
See all Legionella pneumophila products

APPLICATION

Application Note

Immunofluorescent and Immunochemical staining (1:10,000-1:30,000 dilution), Immunohistochemistry (1:3,000-1:10,000), ELISA (1:10,000-1:30,000 dilution), Agglutination (1:2,000-1:5,000)

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Formaldehide treated whole cells of Legionella pneumophila strain Philadelphia 1. Immunized 7 times at two weeks intervals.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Legionella pneumophila antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64135

GTX64135 ICC/IF Image

Immunofluorescent staining of Legionella pneumophila in the infected HEK293 cells using anti-Legionella pneumophila antibody. HEK293 cells were infected with Legionella pneumophila strain Philadelphia1 fixed with 4% formaldehyde and reacted with the anti-Legionella pneumophila antibody at 1/10,000 dilution. As the second antibody, goat Rodamine Red X conjugated anti-rabbit IgG antibody was used at 1/10,000 dilution. DNA was stained with Hoechst 33342 (center) and the images were merged with that of differential interference contrast microscope (right).

Anti-Legionella pneumophila antibody used in ELISA (ELISA). GTX64135

GTX64135 ELISA Image

Titration of antibody reactivity of anti-Legionella pneumophila antiserum by direct ELISA Plate was coated with 100 μl of 109 cells/ml per well and 100 μl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate. "pre" is preimmune serum and "Immunized" is immunized serum

REFERENCE

There are currently no references for Legionella pneumophila antibody (GTX64135). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Legionella pneumophila antibody (GTX64135). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399