Liprin alpha 1 antibody [N1N2], N-term

Cat. No. GTX115098

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr, IP, IHC
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Liprin alpha 1 products

View product citations for antibody GTX115098 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-Fr	Assay dependent
IP	1:100-1:500
IHC	Assay dependent

Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

Neuro2A , Jurkat , 293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Liprin alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624724

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PTPRF interacting protein alpha 1 , LIP.1 , LIP1 , LIPRIN

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Gene ID: 8500 PPFIA1
UniProt: Q13136 PPFIA1

Research Area

DATA IMAGES

GTX115098 WB Image

Liprin alpha 1 antibody detects Liprin alpha 1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Liprin alpha 1 antibody (GTX115098) diluted by 1:1000.

GTX115098 WB Image

Liprin alpha 1 antibody [N1N2], N-term detects PPFIA1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
5 % SDS-PAGE
Liprin alpha 1 antibody [N1N2], N-term (GTX115098) dilution: 1:1000

GTX115098 ICC/IF Image

Liprin alpha 1 antibody [N1N2], N-term detects Liprin alpha 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Liprin alpha 1 stained by Liprin alpha 1 antibody [N1N2], N-term (GTX115098) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115098 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
5% SDS PAGE
GTX115098 diluted at 1:10000

GTX115098 IP Image

Immunoprecipitation of Liprin alpha 1 protein from A431 whole cell extracts using 5 μg of Liprin alpha 1 antibody [N1N2], N-term (GTX115098).
Western blot analysis was performed using Liprin alpha 1 antibody [N1N2], N-term (GTX115098).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX115098 IHC-Fr Image

The data was published in the journal Sci Rep in 2017.PMID: 28831123

GTX115098 IHC-Fr Image

The data was published in the journal Sci Rep in 2017.PMID: 28831123

GTX115098 IHC-Fr Image

The data was published in the journal Sci Rep in 2017.PMID: 28831123

REFERENCE

Application Reference

Bernadzki KM et al. Sci Rep 2017; 7 (1) : 9116 Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery.

Application : IHC

Reactivity : Mouse

Gingras J et al. J Cell Sci 2016; 129 (5) : 898-911 α-Dystrobrevin-1 recruits Grb2 and α-catulin to organize neurotransmitter receptors at the neuromuscular junction.

Application : WB

Reactivity : Mouse

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REVIEW

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Application Reference

Bernadzki KM et al. Sci Rep 2017; 7 (1):9116 Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery.

Application : IHC

Reactivity : Mouse

Gingras J et al. J Cell Sci 2016; 129 (5):898-911 α-Dystrobrevin-1 recruits Grb2 and α-catulin to organize neurotransmitter receptors at the neuromuscular junction.

Application : WB

Reactivity : Mouse

SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

Liprin alpha 1 antibody [N1N2], N-term

Cat. No. GTX115098

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX115098 WB Image

GTX115098 WB Image

GTX115098 ICC/IF Image

GTX115098 WB Image

GTX115098 IP Image

GTX115098 IHC-Fr Image

GTX115098 IHC-Fr Image

GTX115098 IHC-Fr Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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