GeneTex

  • Country / Location Selection

United States (US)

M13 antibody [E1] (Biotin)

Anti-M13 antibody [E1] (Biotin) used in ELISA (ELISA). GTX17269
Anti-M13 antibody [E1] (Biotin) used in Flow cytometry (FACS). GTX17269

Cat. No. GTX17269

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 E1

Isotype

 IgG2a

Application

 WB, FACS, ELISA, IHC

Reactivity

 Bacteriophage M13
Package
50 μg ($399)
See all M13 products

APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For FACS: Use 1 μg/10^6 cells. For WB: Use at a concentration of 1-5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.

Product Note

M13 antibody [E1] (GTX17269) is specific for gp3 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.08% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length M13 phage coat protein; Exact epitope not determined.

Purification

Protein A purified
M13 antibody [E1] (GTX17269) is purified using 0.2μm sterile filter.

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

M13 antibody [E1] (GTX17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.

Research Area

DATA IMAGES

Anti-M13 antibody [E1] (Biotin) used in ELISA (ELISA). GTX17269

GTX17269 ELISA Image

Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.

Anti-M13 antibody [E1] (Biotin) used in Flow cytometry (FACS). GTX17269

GTX17269 FACS Image

Colo320 cell line stained with a cell-line specific M13 phage antibody (filled profile), compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles, then detected with 10ug/ml anti-M13 antibody followed by rabbit anti-mouse IgG:FITC

REFERENCE

There are currently no references for M13 antibody [E1] (Biotin) (GTX17269). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for M13 antibody [E1] (Biotin) (GTX17269). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399