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MAD2L1 antibody [4D2]

Anti-MAD2L1 antibody [4D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84169
Anti-MAD2L1 antibody [4D2] used in Western Blot (WB). GTX84169

Cat. No. GTX84169

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D2

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($519)
See all MAD2L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MAD2L1 (NP_002349) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitotic arrest deficient 2 like 1 , HSMAD2 , MAD2

Cellular Localization

Nucleus,Cytoplasm

Background

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

Database

Research Area

DATA IMAGES

Anti-MAD2L1 antibody [4D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84169

GTX84169 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with MAD2L1 plasmid using GTX84169 MAD2L1 antibody [4D2].

Anti-MAD2L1 antibody [4D2] used in Western Blot (WB). GTX84169

GTX84169 WB Image

WB analysis of HEK293T cells transfected with MAD2L1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84169 MAD2L1 antibody [4D2].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519