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MAD2L1 antibody [GT1761]

KO/KD
Anti-MAD2L1 antibody [GT1761] used in Western Blot (WB). GTX629697
Anti-MAD2L1 antibody [GT1761] used in Western Blot (WB). GTX629697

Cat. No. GTX629697

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1761

Isotype

 IgG2a

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAD2L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:3000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

Jurkat , Raji , 293T

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MAD2L1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888133

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitotic arrest deficient 2 like 1 , HSMAD2 , MAD2

Cellular Localization

Nucleus , Kinetochore , Cytoplasm

Background

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MAD2L1 antibody [GT1761] used in Western Blot (WB). GTX629697

GTX629697 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [GT1761] (GTX629697) diluted at 1:500.

Anti-MAD2L1 antibody [GT1761] used in Western Blot (WB). GTX629697

GTX629697 WB Image

MAD2L1 antibody [GT1761] detects MAD2L1 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
12 % SDS-PAGE
MAD2L1 antibody [GT1761] (GTX629697) dilution: 1:250

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations