APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Heart Tissue Lysate(GTX27932) , Human Heart Tissue Slide
Product Note
This antibody is specific for MAK10 Internal
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
MAK10 antibody was raised against a 14 amino acid synthetic peptide near the center of human MAK10.The immunogen is located within amino acids 420 - 470 of MAK10.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
N(alpha)-acetyltransferase 35, NatC auxiliary subunit , EGAP , MAK10 , MAK10P , bA379P1.1
Cellular Localization
Cytoplasm
Background
The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.
Database
DATA IMAGES
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GTX85264 WB Image
WB analysis of mouse heart tissue lysate using GTX85264 MAK10 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX85264 IHC-P Image
IHC-P analysis of human heart tissue using GTX85264 MAK10 antibody.
Working concentration : 5 μg/ml
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REFERENCE
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REVIEW
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