MARCKS (phospho Ser152) antibody

Cat. No. GTX79020

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Mouse
Package	100 μg ($399)

See all MARCKS products

Datasheet

View product citations for antibody GTX79020 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This antibody is raised against phopsho peptide of human MARCKS Ser158, which shares 100% identity identity with rat MARCKS around Ser152.

Predict Reactivity

Human, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-Sp-F-K).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11164256

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MACS,MARCKS,PKCSL,PRKCSL,myristoylated alanine rich protein kinase C substrate,80KL

Cellular Localization

Cytoplasm,Cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4082 MARCKS
UniProt: P29966 MARCKS

Research Area

DATA IMAGES

GTX79020 WB Image

WB analysis of starved NIH/3T3 cell lysate using GTX79020 MARCKS (phospho Ser152) antibodyy (Lane 3 and 4) and MARCKS antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty