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MASPIN antibody

KO/KD
Anti-MASPIN antibody used in Western Blot (WB). GTX100471
Anti-MASPIN antibody used in Western Blot (WB). GTX100471
Anti-MASPIN antibody used in Western Blot (WB). GTX100471
Anti-MASPIN antibody used in IHC (Paraffin sections) (IHC-P). GTX100471

Cat. No. GTX100471

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MASPIN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLaS3 , NIH-3T3 , HeLa

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MASPIN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951872

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family B member 5 , PI5 , maspin

Cellular Localization

Secreted , extracellular space

Background

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Database

Research Area

DATA IMAGES

Anti-MASPIN antibody used in Western Blot (WB). GTX100471

GTX100471 WB Image

KO/KD

Wild-type (WT) and MASPIN knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MASPIN antibody (GTX100471) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MASPIN antibody used in Western Blot (WB). GTX100471

GTX100471 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
10% SDS PAGE
GTX100471 diluted at 1:1000

Anti-MASPIN antibody used in Western Blot (WB). GTX100471

GTX100471 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX100471 diluted at 1:1000

Anti-MASPIN antibody used in IHC (Paraffin sections) (IHC-P). GTX100471

GTX100471 IHC-P Image

MASPIN antibody detects SERPINB5 protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
MASPIN antibody (GTX100471) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Cheng Y et al. EBioMedicine 2019; 40 : 382-393 Identification of candidate diagnostic and prognostic biomarkers for pancreatic carcinoma.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169