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MAST4 antibody

Anti-MAST4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87899
Anti-MAST4 antibody used in IHC (Paraffin sections) (IHC-P). GTX87899
Anti-MAST4 antibody used in Western Blot (WB). GTX87899
Anti-MAST4 antibody used in Western Blot (WB). GTX87899
Anti-MAST4 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX87899
Anti-MAST4 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX87899

Cat. No. GTX87899

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Monkey
Package
100 μg ($399)
See all MAST4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

284 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human MAST4 (251-300).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10734235

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MAST4 , microtubule associated serine/threonine kinase family member 4

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

Database

Research Area

DATA IMAGES

Anti-MAST4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87899

GTX87899 ICC/IF Image

ICC/IF analysis of COS7 cells using GTX87899 MAST4 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAST4 antibody used in IHC (Paraffin sections) (IHC-P). GTX87899

GTX87899 IHC-P Image

IHC-P analysis of human testis tissue using GTX87899 MAST4 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAST4 antibody used in Western Blot (WB). GTX87899

GTX87899 WB Image

WB analysis of Jurkat, COLO, HUVEC, and MCF-7 cell lysates using GTX87899 MAST4 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-MAST4 antibody used in Western Blot (WB). GTX87899

GTX87899 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 29066835

Anti-MAST4 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX87899

GTX87899 WB IP Image

The data was published in the journal Sci Rep in 2017. PMID: 29066835

Anti-MAST4 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX87899

GTX87899 WB IP Image

The data was published in the journal Sci Rep in 2017. PMID: 29066835

REFERENCE

Application Reference

Gongol B et al. Sci Rep 2017; 7 (1) : 13898 Cellular hormetic response to 27-hydroxycholesterol promotes neuroprotection through AICD induction of MAST4 abundance and kinase activity.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399