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MBD2 antibody [N2C3]

Anti-MBD2 antibody [N2C3] used in Western Blot (WB). GTX105622

Cat. No. GTX105622

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MBD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji

Predict Reactivity

Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MBD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950891

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

methyl-CpG binding domain protein 2 , DMTase , NY-CO-41

Cellular Localization

Nucleus

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MBD2 antibody [N2C3] used in Western Blot (WB). GTX105622

GTX105622 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: JurKat
C: Raji
10% SDS PAGE
GTX105622 diluted at 1:1000

REFERENCE

Application Reference

L Zhang et al. Pharmacogn Mag 2024; RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs
Day RN et al. PLoS One 2021; 16 (12) : e0261660 Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells.
Application : WB
Reactivity : Mouse
Wijenayake S et al. J Comp Physiol B 2016; 186 (3) : 333-42 The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169