APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 2-4μg/ml |
|
Note :
IHC-P
Human Colon shows nuclear stianing of enterocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89182-PEP).
Product Note
This antibody is expected to resognize isoform 1 (NP_003918.1) only.
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
methyl-CpG binding domain protein 2 , DMTase , NY-CO-41
Cellular Localization
Nucleus
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX89182 IHC-P Image
IHC-P analysis of human skin tissue using GTX89182 MBD2 (isoform 1) antibody, Internal.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 2.5μg/ml
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89182 IHC-P Image
IHC-P analysis of human adrenal gland tissue using GTX89182 MBD2 (isoform 1) antibody, Internal.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 2.5μg/ml
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89182 IHC-P Image
IHC-P analysis of human colon using GTX89182 MBD2 (isoform 1) antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89182 WB Image
WB analysis of Jurkat cell lysate using GTX89182 MBD2 (isoform 1) antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for MBD2 (isoform 1) antibody, Internal (GTX89182). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for MBD2 (isoform 1) antibody, Internal (GTX89182). Be the first to share your experience with this product.
