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MCT1 antibody

Orthogonal
Anti-MCT1 antibody used in Western Blot (WB). GTX35258
Anti-MCT1 antibody used in Western Blot (WB). GTX35258
Anti-MCT1 antibody used in Flow cytometry (FACS). GTX35258
Anti-MCT1 antibody used in Western Blot (WB). GTX35258
Anti-MCT1 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX35258

Cat. No. GTX35258

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, FACS, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all MCT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)GRLNDMYGDYKYT, corresponding to amino acid residues 403-415 (6th  extracellular loop) of rat MCT1 (Accession  : P53987).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 16 member 1 , MCT1 , RATMCT1 , RNMCT1

Cellular Localization

Cell membrane

Background

transports lactic acid and other monocarboxylic acids [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-MCT1 antibody used in Western Blot (WB). GTX35258

GTX35258 WB Image

IHC-Frfl (free floating) analysis of mouse hippocampus tissue using GTX35258 MCT1 antibody. Cell nuclei are stained with DAPI (blue).
Panel A : MCT1 staining (red) in CA1 hippocampal region, is detected in the pyramidal layer (P) and in small cell outlines (arrows).
Panel B : GFAP staining (green) is observed in astrocyte outlines (arrows).
Panel C : Merge of the two images demonstrates co-localization of MCT1 and GFAP in astrocytes (arrows).
Dilution : 1:200

Anti-MCT1 antibody used in Western Blot (WB). GTX35258

GTX35258 WB Image

WB analysis of HT-29 (lanes 1 and 3) and ARPE-19 (lanes 2 and 4) cell lysates using GTX35258 MCT1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-MCT1 antibody used in Flow cytometry (FACS). GTX35258

GTX35258 FACS Image

FACS analysis of Jurkat cells using GTX35258 MCT1 antibody.
Black : Unstained cell
Red : Cell staining with isotype control antibody
Green : Cell staining with primary antibody
Antibody amount : 5 μg

Anti-MCT1 antibody used in Western Blot (WB). GTX35258

GTX35258 WB Image

WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)  brain lysates using GTX35258 MCT1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-MCT1 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX35258

GTX35258 IHC (Free Floating) Image

WB analysis of rat skeletal muscle lysate using GTX35258 MCT1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

There are currently no references for MCT1 antibody (GTX35258). Be the first to share your publications with this product.
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REVIEW

MCT1 antibody Cat. No. GTX35258

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-MCT1 antibody used in Flow cytometry (FACS). GTX35258
Date : Anonymous submitted on 25-May-2021
Score :
Application Tested : FACS
Sample Species : Hu
Sample : A549 cells
Primary Antibody : 1:100, 4°C, 1Hr
Anti-MCT1 antibody used in Western Blot (WB). GTX35258
Date : Anonymous submitted on 25-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A375 cells
Blocking : 5% BSA in TBST, 37°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639