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MDFIC antibody, N-term

Anti-MDFIC antibody, N-term used in Flow cytometry (FACS). GTX81076
Anti-MDFIC antibody, N-term used in Western Blot (WB). GTX81076
Anti-MDFIC antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81076

Cat. No. GTX81076

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all MDFIC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 73-99 amino acids from the N-terminal region of human MDFIC.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MyoD family inhibitor domain containing , HIC , MDFIC1

Background

This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-MDFIC antibody, N-term used in Flow cytometry (FACS). GTX81076

GTX81076 FACS Image

FACS analysis of MCF-7 cells using GTX81076 MDFIC antibody, N-term.
Top histogram : negative control
Bottom histogram : MCF-7 cells

Anti-MDFIC antibody, N-term used in Western Blot (WB). GTX81076

GTX81076 WB Image

WB analysis of HeLa cell lysate (35ug/lane) using GTX81076 MDFIC antibody, N-term.

Anti-MDFIC antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81076

GTX81076 IHC-P Image

IHC-P analysis of human spleen using GTX81076 MDFIC antibody, N-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399